Bakota, Martin2023-06-072023-06-071991https://unbscholar.lib.unb.ca/handle/1882/34442A patch-clamping system consisting of a computer controlled pA amplifier and signal averager was designed to measure currents through single ionic channels. Techniques were established for reliable micropipette (tip 0.0. <2μ) manufacture and tungsten microelectrode use in patch-clamping. Attempts were made to measure single channel currents in giant axons of crayfish (Orconectes). These were unsuccessful due to a failure to establish GΩ seals between the pipette and the plasma membrane. The failure is attributed to unclean plasma membrane surface (it was not digested with proteolytic enzymes).42 pageselectronicen-CAhttp://purl.org/coar/access_right/c_16ecsenior reportSastry, K.V.L.N.Physics