Antimycobacterial natural products from Heracleum maximum

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dc.contributor.advisorGray, Christopher
dc.contributor.authorO'Neill, Taryn Elaine
dc.date.accessioned2023-03-01T16:45:17Z
dc.date.available2023-03-01T16:45:17Z
dc.date.issued2012
dc.date.updated2020-08-11T00:00:00Z
dc.description.abstractTuberculosis is a major global health problem that has limited therapeutic options available. N atural [natural] products have historically p rovided [provided] the majority of anti-infective agents, and represent a promising source of antimycobacterial compounds. The objective of this research was to identify the bioactive constituents of a traditional medicinal plant, Heracleum maximum, through fractionation guided by the microplate resazurin assay (MRA). However, when we tested the methanolic extracts of Heracleum maximum, we found the MRA to be problematic, providing inconsistent results. The conditions of the MRA were optimised to provide a standard screening protocol for use in screening and bioassay-guided fractionation of methanolic plant extracts. The optimised MRA was then used to isolate eight antimycobacterial compounds from the Heracleum maximum extract. Among the isolated compounds the polyacetylene, (3R,8S)-falcarindiol, and the furanocoumarin, 6-isopentenyloxyisobergapten, expressed the greatest inhibitory activities; whereas the remaining furanocoumarins, bergapten, isobergapten, angelicin, sphondin, pimpinellin, and isopimpinellin exhibited only modest activities.
dc.description.copyright©Taryn O'Neill, 2012
dc.description.noteScanned from archival print submission.
dc.formattext/xml
dc.format.extentxx, 181 pages
dc.format.mediumelectronic
dc.identifier.oclc(OCoLC)1341641393en
dc.identifier.otherThesis 9011
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/14433
dc.language.isoen_CA
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineBiology
dc.subject.lcshAnti-infective agents.en
dc.subject.lcshParsnip.en
dc.subject.lcshMicroplates.en
dc.subject.lcshTuberculosis--Microbiology.en
dc.titleAntimycobacterial natural products from Heracleum maximum
dc.typemaster thesis
thesis.degree.disciplineBiology
thesis.degree.fullnameMaster of Science
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.Sc.

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