Make new methods but keep the old: Exploring the use of DNA analysis to study stress in past populations

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dc.contributor.advisorScott, Amy
dc.contributor.advisorAddison, Jason
dc.contributor.authorBrien, Natassja
dc.date.accessioned2024-01-24T18:46:33Z
dc.date.available2024-01-24T18:46:33Z
dc.date.issued2023-08
dc.description.abstractThe main aim of this project is to develop and adapt a method currently used in clinical research, DNA methylation analysis of a gene target, to assess stress in past human populations using ancient DNA (aDNA). Specifically, this work will focus on three goals: a) extracting and purifying aDNA from an archaeological source, b) developing a method to amplify and sequence the NR3C1 gene (which encodes the glucocorticoid receptor), including the design and testing of primers that successfully target the gene and optimizing a PCR protocol for use with the primers, and c) applying this method to aDNA extracted from archaeological sources. This preliminary work will allow future researchers to assess differences in methylation levels of NR3C1 and to ascertain whether methylation of that locus can be used to gain additional information on stress in past populations (González Ramírez et al., 2020; Liu & Nusslock, 2017; McGowan et al., 2009).
dc.description.copyright©Natassja Brien, 2023
dc.format.extentx, 111
dc.format.mediumelectronic
dc.identifier.oclc(OCoLC)1427566769en
dc.identifier.otherThesis 11217en
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/37666
dc.language.isoen
dc.publisherUniversity of New Brunswick
dc.relationSocial Sciences and Humanities Research Council of Canada
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineInterdisciplinary Studies
dc.subject.lcshMolecular genetics.en
dc.subject.lcshStress (Physiology)en
dc.subject.lcshDNA, Fossil.en
dc.subject.lcshMethylation.en
dc.titleMake new methods but keep the old: Exploring the use of DNA analysis to study stress in past populations
dc.typemaster thesis
oaire.license.conditionother
thesis.degree.disciplineInterdisciplinary Studies
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.IDST.

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