Isolation and identification of the bioactive natural product A-26771B from an endophytic Penicillium expansum isolate
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Date
2020
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University of New Brunswick
Abstract
Natural products are an important source of bioactive compounds and endophytic fungi derived from marine algae represent an under-investigated source of natural products. The objective of this research was to isolate and confirm that an extract from an endophytic Penicillium expansum contained the natural product A-26771B. The natural product was tentatively detected, using high-resolution electrospray ionization mass spectroscopy data, in a methanolic extract from the endophyte. Despite repeated rounds of high performance liquid chromatography and multiple fermentations of the endophyte, the compound was isolated in sub milligram quantities and identified using proton nuclear magnetic resonance data. However, prior to obtaining carbon nuclear magnetic resonance data that would confirm the identification of A-26771B the natural product degraded. Future work should include isolating more of the molecule A-26771B to determine the bioactivity of the molecule against Mycobacterium tuberculosis and Mycobacterium smegmatis.