A patch clamp system for cell-attached recordings from crayfish giant axons

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dc.contributor.advisorSastry, K.V.L.N.
dc.contributor.authorBakota, Martin
dc.date.accessioned2023-06-07T20:32:42Z
dc.date.available2023-06-07T20:32:42Z
dc.date.issued1991
dc.description.abstractA patch-clamping system consisting of a computer controlled pA amplifier and signal averager was designed to measure currents through single ionic channels. Techniques were established for reliable micropipette (tip 0.0. <2μ) manufacture and tungsten microelectrode use in patch-clamping. Attempts were made to measure single channel currents in giant axons of crayfish (Orconectes). These were unsuccessful due to a failure to establish GΩ seals between the pipette and the plasma membrane. The failure is attributed to unclean plasma membrane surface (it was not digested with proteolytic enzymes).
dc.description.copyrightNot available for use outside of the University of New Brunswick
dc.description.note4unnumbered pages before first numbered page
dc.format.extent42 pages
dc.format.mediumelectronic
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/34442
dc.language.isoen_CA
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subject.disciplinePhysics
dc.titleA patch clamp system for cell-attached recordings from crayfish giant axons
dc.typesenior report
thesis.degree.disciplinePhysics
thesis.degree.fullnameBachelor of Science
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelundergraduate
thesis.degree.nameB.Sc.

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