The application of DNA taxonomy to identify fungal endophytes

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dc.contributor.advisorGray, Christopher
dc.contributor.authorStack, Ryleigh Anne Jean
dc.date.accessioned2023-03-01T16:21:11Z
dc.date.available2023-03-01T16:21:11Z
dc.date.issued2020
dc.date.updated2023-03-01T15:01:48Z
dc.description.abstractFungal identification has been a long-standing challenge in mycology. DNA barcoding and DNA taxonomy have been found to mitigate challenges associated with fungal identification, in this project these techniques were compared. The Natural Products Research Group (NPRG) has a collection of over 400 endophytes, 242 of which had been identified using DNA barcoding, resulting in ambiguous or missing identifications for approximately 25% of the isolates. In this study, DNA taxonomy was used to resolve and verify identifications in the NPRG's collection. To determine if DNA taxonomy is useful in identifying new DNA sequences, a test set of endophytes was isolated and a phylogenetic tree of 441 sequences was built. Analysis of this tree resulted in 55 sequences from the NPRG collection being identified (45) or re-identified (10), improving the collection's identifications. Finally, 47 of the new sequences (76%) were identified to the species (4), genus (42), or order (1) level.
dc.description.copyright© Ryleigh A. Stack, 2021
dc.formattext/xml
dc.format.extentix, 150 pages
dc.format.mediumelectronic
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/13611
dc.language.isoen_CA
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineBiology
dc.titleThe application of DNA taxonomy to identify fungal endophytes
dc.typemaster thesis
thesis.degree.disciplineBiology
thesis.degree.fullnameMaster of Science
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.Sc.

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