A suppressor mutation approach to determining the sequence requirements of an unusual two-subunit sigma factor and its cognate promoter element
| dc.contributor.advisor | MacLellan, Shawn | |
| dc.contributor.author | Kesthely, Christopher, Andrew | |
| dc.date.accessioned | 2023-03-01T16:18:25Z | |
| dc.date.available | 2023-03-01T16:18:25Z | |
| dc.date.issued | 2017 | |
| dc.date.updated | 2023-03-01T15:01:28Z | |
| dc.description.abstract | Transcription is a highly-regulated process. In bacteria, σ factors are transcription factors that regulate RNA polymerase binding to promoters, and the conversion of double stranded DNA to the single-stranded state required for the initiation of RNA synthesis. The Bacillus subtilis genome encodes an atypical, twosubunit σ factor called SigO-RsoA. To better understand the genes regulated by this σ factor, I conducted the largest phylogenetic analysis of the SigO-RsoA regulon to date and compiled and analyzed the sequences of 216 orthologous target promoters. A comprehensive mutational analysis of a conserved thymine trinucleotide and a -10 promoter element cognate to SigO-RsoA delineated key nucleotides required for the activity of this σ factor. Selected inactive promoter mutants were used in a suppressor mutation approach to identify whether it is SigO or RsoA that is responsible for interaction with the key -10 promoter element. | |
| dc.description.copyright | ©Christopher Andrew Kesthely, 2018 | |
| dc.format | text/xml | |
| dc.format.extent | xiii, 137 pages | |
| dc.format.medium | electronic | |
| dc.identifier.uri | https://unbscholar.lib.unb.ca/handle/1882/13423 | |
| dc.language.iso | en_CA | |
| dc.publisher | University of New Brunswick | |
| dc.rights | http://purl.org/coar/access_right/c_abf2 | |
| dc.subject.discipline | Biology | |
| dc.title | A suppressor mutation approach to determining the sequence requirements of an unusual two-subunit sigma factor and its cognate promoter element | |
| dc.type | master thesis | |
| thesis.degree.discipline | Biology | |
| thesis.degree.fullname | Master of Science | |
| thesis.degree.grantor | University of New Brunswick | |
| thesis.degree.level | masters | |
| thesis.degree.name | M.Sc. |
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