A suppressor mutation approach to determining the sequence requirements of an unusual two-subunit sigma factor and its cognate promoter element

dc.contributor.advisorMacLellan, Shawn
dc.contributor.authorKesthely, Christopher, Andrew
dc.date.accessioned2023-03-01T16:18:25Z
dc.date.available2023-03-01T16:18:25Z
dc.date.issued2017
dc.date.updated2023-03-01T15:01:28Z
dc.description.abstractTranscription is a highly-regulated process. In bacteria, σ factors are transcription factors that regulate RNA polymerase binding to promoters, and the conversion of double stranded DNA to the single-stranded state required for the initiation of RNA synthesis. The Bacillus subtilis genome encodes an atypical, twosubunit σ factor called SigO-RsoA. To better understand the genes regulated by this σ factor, I conducted the largest phylogenetic analysis of the SigO-RsoA regulon to date and compiled and analyzed the sequences of 216 orthologous target promoters. A comprehensive mutational analysis of a conserved thymine trinucleotide and a -10 promoter element cognate to SigO-RsoA delineated key nucleotides required for the activity of this σ factor. Selected inactive promoter mutants were used in a suppressor mutation approach to identify whether it is SigO or RsoA that is responsible for interaction with the key -10 promoter element.
dc.description.copyright©Christopher Andrew Kesthely, 2018
dc.formattext/xml
dc.format.extentxiii, 137 pages
dc.format.mediumelectronic
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/13423
dc.language.isoen_CA
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineBiology
dc.titleA suppressor mutation approach to determining the sequence requirements of an unusual two-subunit sigma factor and its cognate promoter element
dc.typemaster thesis
thesis.degree.disciplineBiology
thesis.degree.fullnameMaster of Science
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.Sc.

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