The effects of ethinylestradiol (EE2) on molecular signalling cascades in mummichog (Fundulus heteroclitus)

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dc.contributor.advisorMartyniuk, Chris
dc.contributor.advisorMunkittrick, Kelly
dc.contributor.authorDoyle, Meghan A.
dc.date.accessioned2023-03-01T16:30:20Z
dc.date.available2023-03-01T16:30:20Z
dc.date.issued2012
dc.date.updated2020-07-22T00:00:00Z
dc.description.abstractExposures to ≤10 ng/L of 17 α-ethinylestradiol (EE2), a synthetic estrogen used in birth control pills, will reduce or shutdown egg production in freshwater fish models, while mummichog (Fundulus heteroclitus), an estuarine species, are able to produce eggs at EE2 concentrations of >3000 ng/L. The objective of this research was to gain mechanistic insights into how mummichogs are able to produce eggs at high EE2 exposures. Mummichog were exposed to 0, 50 or 250 ng/L of EE2 for 14 d. There were no differences in gonadosomatic index, liversomatic index, gonad development, or plasma estradiol levels. However, testosterone decreased with increasing EE2 concentrations (50, 250 ng/L). Microarray analysis of liver tissue revealed that cell processes associated with apoptosis, lipids, and translation were affected by EE2. Transcriptomics data are comparable to other fish studies and the liver appears to be under generalized stress from EE2 exposure. This study suggests that mummichog are able to maintain lipid/vitellogenin uptake by the ovary despite exposure to EE2, while other species are unable to do so.
dc.description.copyright©M.A. Doyle, 2012
dc.description.noteScanned from archival print submission.
dc.formattext/xml
dc.format.extentx, 98 pages
dc.format.mediumelectronic
dc.identifier.oclc(OCoLC)1317828463en
dc.identifier.otherThesis 9034
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/13993
dc.language.isoen_CA
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineBiology
dc.subject.lcshEstrogen.en
dc.subject.lcshMummichog.en
dc.subject.lcshFishes--Eggs.en
dc.subject.lcshTestosterone.en
dc.titleThe effects of ethinylestradiol (EE2) on molecular signalling cascades in mummichog (Fundulus heteroclitus)
dc.typemaster thesis
thesis.degree.disciplineBiology
thesis.degree.fullnameMaster of Science
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.Sc.

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