Investigating the molecular basis for autorepression of a gene promoter from the α-proteobacterium Sinorhizobium meliloti
| dc.contributor.advisor | MacLellan, Shawn R. | |
| dc.contributor.author | Kuang, ZiYu | |
| dc.date.accessioned | 2024-10-22T16:55:06Z | |
| dc.date.available | 2024-10-22T16:55:06Z | |
| dc.date.issued | 2024-08 | |
| dc.description.abstract | Autorepression is an intrinsic pathway of autoregulation developed to maintain homeostasis in biological systems. The srlA gene encodes a predicted thioredoxin-like protein and deletion of srlA results in increased sensitivity to salt and oxidizing agents in the nitrogen-fixing facultative endosymbiont, Sinorhizobium meliloti. The srlA promoter is autorepressed by an unknown mechanism. In my thesis, I investigate the mechanism of the autorepression phenotype. Chapter 1 is a literature review and provides an overview of the srlA promoter and gene, the autorepression phenotype observed, as well as possible autorepression mechanisms. Chapter 2 documents a bioinformatic analysis of SrlA protein structure and possible subcellular location, and an experimental analysis of whether the thioredoxin-like C-X-X-C motif of SrlA is required for the autorepression phenotype. Chapter 3 details the use of transposon mutagenesis and a promoter activation screen to investigate the possibility that other S. meliloti genes play a role in the autorepression of srlA. Chapter 4 investigates whether gene srlA is required for effective nodulation and growth stimulation of the S. meliloti plant host, alfalfa (Medicago sativa). Finally, Chapter 5 includes a summary of the thesis and concluding statements. | |
| dc.description.copyright | © ZiYu Kuang, 2024 | |
| dc.format.extent | xii, 107 | |
| dc.format.medium | electronic | |
| dc.identifier.uri | https://unbscholar.lib.unb.ca/handle/1882/38168 | |
| dc.language.iso | en | |
| dc.publisher | University of New Brunswick | |
| dc.rights | http://purl.org/coar/access_right/c_abf2 | |
| dc.subject.discipline | Biology | |
| dc.title | Investigating the molecular basis for autorepression of a gene promoter from the α-proteobacterium Sinorhizobium meliloti | |
| dc.type | master thesis | |
| oaire.license.condition | other | |
| thesis.degree.discipline | Biology | |
| thesis.degree.grantor | University of New Brunswick | |
| thesis.degree.level | masters | |
| thesis.degree.name | M.Sc. |