Investigating the molecular basis for autorepression of a gene promoter from the α-proteobacterium Sinorhizobium meliloti

dc.contributor.advisorMacLellan, Shawn R.
dc.contributor.authorKuang, ZiYu
dc.date.accessioned2024-10-22T16:55:06Z
dc.date.available2024-10-22T16:55:06Z
dc.date.issued2024-08
dc.description.abstractAutorepression is an intrinsic pathway of autoregulation developed to maintain homeostasis in biological systems. The srlA gene encodes a predicted thioredoxin-like protein and deletion of srlA results in increased sensitivity to salt and oxidizing agents in the nitrogen-fixing facultative endosymbiont, Sinorhizobium meliloti. The srlA promoter is autorepressed by an unknown mechanism. In my thesis, I investigate the mechanism of the autorepression phenotype. Chapter 1 is a literature review and provides an overview of the srlA promoter and gene, the autorepression phenotype observed, as well as possible autorepression mechanisms. Chapter 2 documents a bioinformatic analysis of SrlA protein structure and possible subcellular location, and an experimental analysis of whether the thioredoxin-like C-X-X-C motif of SrlA is required for the autorepression phenotype. Chapter 3 details the use of transposon mutagenesis and a promoter activation screen to investigate the possibility that other S. meliloti genes play a role in the autorepression of srlA. Chapter 4 investigates whether gene srlA is required for effective nodulation and growth stimulation of the S. meliloti plant host, alfalfa (Medicago sativa). Finally, Chapter 5 includes a summary of the thesis and concluding statements.
dc.description.copyright© ZiYu Kuang, 2024
dc.format.extentxii, 107
dc.format.mediumelectronic
dc.identifier.urihttps://unbscholar.lib.unb.ca/handle/1882/38168
dc.language.isoen
dc.publisherUniversity of New Brunswick
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.disciplineBiology
dc.titleInvestigating the molecular basis for autorepression of a gene promoter from the α-proteobacterium Sinorhizobium meliloti
dc.typemaster thesis
oaire.license.conditionother
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of New Brunswick
thesis.degree.levelmasters
thesis.degree.nameM.Sc.

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